Fig. 3. Inner ears of Shh and Gli3 compound mutants. Paint-filled inner ears of (A) Shh^+/-;Gli3^+/+, (B) Shh^-/-;Gli3^+/+, (C) Shh^-/-;Gli3^+/-, (D) Shh^-/-;Gli3^-/- and (E) Gli3699 mutants at 14.75 dpc. (B) Shh^-/-;Gli3^+/+ inner ears lack ventral structures, including the cochlear duct and saccule (asterisk). Dorsally, the lateral semicircular canal/ampulla and endolymphatic duct are missing. (C) The dorsal structures in Shh^-/-;Gli3^+/- inner ears are normal, but only a small knob-like structure is present ventrally (arrow). (D) In Shh^-/-;Gli3^-/- ears, the dorsal structures are malformed, and a shortened cochlear duct is present. (E) Inner ears of Gli3699 homozygotes show normal dorsal inner ear phenotypes, yet the cochlear duct is shortened. (A',D',E') Ventral views of respective cochlear ducts. For abbreviations refer to Fig. 2; p, proximal; d, distal. Scale bar: 100 µm.