Fig. 8. MASH1 binds to the I12b enhancer in vivo. LGE and MGE were dissected from E11.5 mouse telencephalon, cross-linked and immunoprecipitated using an anti-MASH1 antibody (-Mash1). PCR analysis was performed on the immunoprecipitated chromatin using primers corresponding to the I12b enhancer (I12b Enh) and the Hes6 upstream enhancer (Hes6 Enh). After 28-30 cycles, PCR products (605 bp for mI12b and 176 bp for Hes6) were electrophoresed on 1% agarose gel. As controls, amplifications were carried out on chromatin obtained from the elution of protein-A sepharose beads that was preincubated with the cross-linked chromatin or with the IP dilution buffer (No antibody and Mock, respectively). Different dilutions of total input chromatin (Genomic DNA) were used for each set of primers: 1/500 for I12b and 1/1000 for Hes6 Enh.