Fig. 4. Effect of tup clones on notum genes. tup clones are identified by the absence of GFP (green) expression. (A) Wild-type expression of ush and pnr-Gal4 in the medial notum. Arrowheads, ush expression in the lateral notum and hinge regions. (B) First instarinduced tup^ex4 clone. pnr-Gal4 expression is lost. The Ush accumulation pattern (arrowhead) is similar to that in the hinge area. (C) Second instar-induced tup^ex4 clones. ush is repressed. (D) tup² clones (blue arrowheads) repress pnr-Gal4 (blue or white) and two of the tup² clones upregulate ara/caup (white arrowheads). (E) tup² clones. Only that clone in the central notum (arrow) represses ara/caup (red). Proximal clones upregulate ara/caup (arrowheads). Inset shows that pnr-Gal4 expression (white or blue) persists in most clones. (F) Regions are outlined where tup clones lose (blue, mapped with 13 clones overlapping the area) or gain (white, mapped with 28 clones) ara/caup expression. (G) tup^isl-1 clone. msh-lacZ is upregulated (arrowhead) and ara/caup downregulated (arrow) in the same cells. (H) Anterior tup² clone. ara/caup (arrow) and msh-lacZ (arrowhead) are both upregulated in some cells of the clone. (I) First instar-induced tup^ex4 clone showing derepression of msh-lacZ and inhibition of eyg. Inset shows wild-type expression of eyg.