Fig. 6. Overexpression of tup and ara synergistically promote transformation towards notum. (A-D) Mesothoracic pleurae. Red arrowheads indicate anterior (anp) and posterior (pnp) notopleural, and ventral sternopleural (vsp) bristles; black arrowheads, tegulae (tg); asterisks, vertical clefts. (A) Wild type. (B) UAS-ara; C765-Gal4. (C) UAS-tup/C765-Gal4. Arrow, notum-like outgrowth on the vertical cleft. (D) UAS-ara; UAS-tup/C765-Gal4. Flies were grown at 17°C. Black arrow, notum expansion towards the pleura; wing is missing. White arrow, notum-like structure adjacent to sternopleural bristles (red arrowhead). (E-H) Wing discs showing expression of eyg (green) and DC-lacZ (red). Genotypes in E-H correspond with those shown in A-D, respectively. Arrowhead (G) indicates ectopic expression in the prospective pleura (this location was verified in an optical z-section). (H) eyg is expressed at the dorsal hinge (arrowhead) and the wing and pleura territories (arrows). Insets show red channel images of the pleura and wing pouch areas. (I) UAS-ara; UAS-tup/dpp^blk-Gal4 fly. Notum-like structures form on the central wing (asterisk), pleura (black arrow) and sternopleurite (red arrow). aw and pw, anterior and posterior parts of the wing, respectively. (J) UAS-ara; UAS-tup/dpp^blk-Gal4 mesothoracic leg disc. Arrowhead, ectopic eyg expression. (K-M) Wild-type (L) and UAS-ara; UAS-tup/dpp^blk-Gal4 (K,M) wing discs showing either eyg (K) or nub and zfh2 (L,M) expression. Arrows (K) indicate that eyg expression is expanded to the driver territory. Arrowheads (M) indicate that nub and zfh2 expression is lost from the driver territory.