Fig. 7. Regulation of tup in the wing disc. Red, Tup; blue or white, Ara/Caup. (A) M⁺ Egfr^1K35 clones (absence of green) remove ara/caup expression (arrowheads) but do not inhibit tup (arrows). (B) M⁺ Ras85D^C40b clones (absence of green) inhibit ara/caup (arrowheads), but not tup expression. (C) Second (top row) and early third (bottom row) instar discs. Overexpression of Mkp3 (green) inhibits ara/caup (arrowhead), but not tup (arrow). (D) Expression of ara/caup in wild-type discs of similar age to those shown in C. (E) Clones expressing UAS-Ras1^V12 (green) activate ara/caup (arrowheads) in the wing hinge. tup is not activated, or only so at very low levels. (F) A tkv^a12 clone (absence of green) removes tup expression in the medial (arrowhead), but not in the lateral (arrow) notum. (G) Second (top) and early third (bottom) instar discs. Overexpression of UAS-Dad (green) blocks Tup accumulation (arrowheads). Compare with Fig. 2B,C. (H) Clones expressing UAS-tkv^QD (green) activate tup in the medial (arrowhead), but not in the lateral (arrow) notum.