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Figure 3


Fig. 3. Development of the embryonic metanephros and ureter in Dlgh1-/- mice. Immunofluorescence staining of urinary organs of Dlgh1+/+ (upper panels) and Dlgh1-/- (lower panels) mice with an anti-Pax2 antibody (A,D) or anti-Pax2 (green) and anti-pan-cytokeratin (red) antibodies (B,C). Note that the metanephric Pax2-positive area is contracted in Dlgh1-/- mice. The insets in B and C show staining with an anti-pan-cytokeratin antibody. The pairs of arrowheads delineate the region of the common nephric duct. Md, Müllerian duct; ur, ureteric bud; Wd, Wolffian duct.