Fig. 7. Expression patterns of Dlgh1 and Ecadherin in embryos. (A,B) Expression patterns of Dlgh1 (A) and Pax2 (B) in Dlgh1^+/+ mice at E11.5. Dlgh1 is expressed not only in the Wolffian duct and ureter, which are Pax2-positive, but also in the epithelial cells of the hindgut, which are Pax2-negative. In addition, mesenchymal cells within the metanephric blastema are also weakly Dlgh1-positive. (C-J) Subcellular distributions of Dlgh1 (C,G), E-cadherin (D,H, red in E,F,I,J), occludin (green in E,I) and ZO-1 (green in F,J) in the ureteric epithelium of Dlgh1^+/+ (C-F) and Dlgh1^-/- (G-J) mice at E14.5 (C,D,G,H) and E15.5 (E,F,I,J). C,D and G,H represent each color of the same sections, respectively. In Dlgh1^+/+ mice, Dlgh1 is accumulated at the basolateral region of the ureteric epithelium (C), whereas no significant Dlgh1-positive signals are detected in Dlgh1^-/- mice (G). The subcellular distributions of E-cadherin, occludin and ZO-1 in Dlgh1^-/- mice are similar to those in Dlgh1^+/+ mice; staining of E-cadherin in the basement membrane of frozen sections (E,F,I,J) tended to be more intense than that in paraffin sections (C,D,G,H). hg, hindgut; mn, metanephros; ur, ureter; Wd, Wolffian duct. Scale bars: 100 µm in A,B; 10 µm in C-J.