Fig. 2. Apical wg localization and WLE3 activity is conserved. (A-C) Confocal images of endogenous wg RNA (green) detected by FISH in germ band extended embryos of D. prosaltans (A), D. hydei (B) and Z. tuberculatus (C). Nuclei are red and membranes blue. (D-I) Representative images of D. melanogaster embryos injected with full-length Drosophila wg 3'UTR sequences (D-F), or with chimeric sequences in which the D. melanogaster WLE3 of construct H was replaced by that of D. prosaltans (G), D. hydei (H) or Z. tuberculatus (I). Scale bars: 10 µm.