Fig. 4. Importance of sequence and base-pairing at conserved base-pair positions. (A-C) Diagrams of D. melanogaster WLE3 (A) or just the WLE3 distal stem (B,C). Bases targeted by each mutation are boxed in the wild-type diagram, with arrows pointing to mutant sequences shown to either side (mutant numbers/names are indicated at the bottom). The shading indicates activity: white text on black, no activity (all -); white text on dark grey, weak activity (mostly -); black text on light grey, moderate activity (+); black text on white, full activity (++). To the far right of each diagram are representative images of injected embryos. Scale bars: 10 µm. Base transversions are depicted at the immediate left or right of WLE3, and compensatory mutations further right.