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Figure 2


Fig. 2. Cellular localization of DsRed in coronal sections of P30 tg brain. (A,B) DsRed fluorescence (red) is detected in all the cells that are NG2+ (green) in corpus callosum (A) and cerebral cortex (B). (C) All the cells exhibiting DsRed fluorescence in the cerebral cortex express PDGFR{alpha}+ (green). (D) Comparison of DsRed fluorescence (red) and DsRed protein detected by a rabbit anti-DsRed antibody (green) in the cerebral cortex. DsRed protein is detected in the soma and throughout the processes. (E) Comparison of DsRed fluorescence with the oligodendrocyte antigen APC (green) in the corpus callosum. Strongly DsRed+ cells are negative for APC (arrows), whereas some weakly DsRed+ cells are APC+ (arrowheads). (F,G) DsRed fluorescence is not detected in astrocytes expressing GFAP (F) or S100β (G) in the cerebral cortex. (H) DsRed fluorescence is not detected in F4/80+ microglia in the cerebral cortex. (I) DsRed fluorescence is not detected in NeuN+ neurons in the cerebral cortex. Yellow indicates regions of contact, but the two antigens are not present in the same cells. (J,K) DsRed fluorescence is detected in PDGFRβ+ pericytes (K, arrowheads) but not in CD31+ endothelial cells (J, arrowheads) in the cerebral cortex. Scale bars: 12.5 µm in A-C; 20 µm in D,E; 25 µm in F-K.