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Figure 4


Fig. 4. EGFP is expressed in APC+ oligodendrocytes and myelin in the white matter of P14 NG2CreBAC:Z/EG double tg mouse. Coronal sections (50 µm) through corpus callosum or striatum from P14 double tg mouse were double labeled with rabbit anti-EGFP (A,D,G; green), and mouse anti-APC (B,E,H; red) antibodies. (A-C) Projected images of z-stacks from the corpus callosum. The distribution of EGFP+ cells is similar to that of APC+ cells with extensive overlap between EGFP and APC. (D-I) Higher magnification of projected images of z-stacks from the corpus callosum (D-F) and striatum (G-I). APC+ oligodendrocytes in the corpus callosum express EGFP (straight arrows). EGFP-expressing NG2 glia are negative for APC (arrowheads). Some of the APC+ oligodendrocytes do not express EGFP (wavy arrows). EGFP+ oligodendrocytes form myelin, which is also EGFP+ (asterisks). Scale bars: 20 µm.