Fig. 6. NG2 cell-astrocyte transition in P1 NG2creBAC:Z/EG double tg mice. (A-D) Coronal sections (50 µm) through ventral cortex from a P1 double tg mouse were triple labeled with rabbit anti-EGFP (green in A,D), guinea pig anti-NG2 (red in B,D) and mouse anti-nestin (blue in C,D) antibodies. An EGFP+nestin+ cell (straight arrows) with protoplasmic astrocytic morphology and weak NG2 expression extends a process with a broad end-foot (wavy arrows) that contacts a blood vessel (asterisks). Two typical NG2 glia on the right with strong NG2 expression (arrowheads) do not have vascular end-feet. Scale bars: 20 µm.