Fig. 2. Generation of Hoxc10 and Hoxd10 knockout alleles. (A) Schematic showing the wild-type (WT) genomic loci for Hoxc10 and Hoxd10, as well as targeted alleles for Hoxc10 and Hoxd10. (B) Southern blot analysis of DNA from ES cell clones for Hoxc10^RFP and Hoxd10^hrGFP. For Hoxc10^RFP, ScaI digestion and a 3' flanking probe were used. The WT band is 11.5 kb and the correctly targeted band is 5.8 kb. For Hoxd10^hrGFP, EcoRI digestion and a 3' flanking probe were used. The corresponding WT band is 12.4 kb, and the mutant band is 9.6 kb. (C) A typical hindlimb posture for Hoxc10^-/-/Hoxd10^-/- mutants and WT control.