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Figure 3


Fig. 3. Reduction of JNK and c-Jun phosphorylation in M1+/{Delta}KDJ1-/- eyelid epithelium. (A) The M1+/{Delta}KDJ1-/- and M1+/{Delta}KDJ2-/- fetuses at E15.5 were examined by immunohistochemistry for the phospho- and total JNK and c-Jun, phospho-EGFR (p-EGFR), phospho-ERK (p-ERK) and phospho-Elk (p-Elk). Levels of only p-JNK and p-c-Jun were significantly reduced in the developing eyelid epithelium of the M1+/{Delta}KDJ1-/- fetuses. Scale bars: 50 µm. (B) Quantitative summary of the percentage of p-JNK- and p-c-Jun-positive cells in suprabasal epithelial layers of the developing eyelids in various gene knockout fetuses. Data are mean ± s.d. of at least four fetuses of each genotype examined. Only the M1+/{Delta}KDJ1-/- fetuses showed significant (*P<0.01) reduction in p-JNK and p-c-Jun compared with wild-type fetuses. (C) E15.5 Jnk1-null (J1-/-) and Jnk2-null (J2-/-) fetuses were subjected to X-Gal staining to detect the target gene promoter-driven β-galactosidase expression. X-Gal-positive cells were detected in the entire embryonic body of E15.5 fetuses. Arrowheads indicate the developing eyelid tip.