Fig. 4. Weak Uba1 alleles suppress apoptosis. (A) GMR-hid eye disc labeled with cleaved Caspase-3 (Cas3^*) antibodies. Yellow arrows indicate two zones of dying cells in GMR-hid. (B,B') Suppression of Cas3^*-positive cell death in GMR-hid discs. The yellow arrow indicates the position of the first zone of Cas3^*-positive cell death in GMR-hid. Uba1^D6 clones suppress Cas3^*-positive cell death (white arrows). However, this suppression is not uniform and occurs in a region-specific manner. Clones in an area of the disc marked by the red arrow are still apoptotic. Genotype: GMR-hid ey-FLP; FRT42D Uba1^D6/FRT42D P[ubi-GFP]; (C-C'') Uba1^D6 clones suppress most normal developmental cell death in 28 hours APF pupal discs. Genotype as in B. (D-D'') Uba1^H33 clones contain additional IOCs in 42 hours APF pupal discs. Genotype: ey-FLP; FRT42D Uba1^H33/FRT42D P[ubi-GFP]. (E-E'') Diap1 protein levels are increased in Uba1^H33 mutant clones (arrows). Genotype as in D. (F-F'') Dronc protein levels are decreased in Uba1^D6 mutant clones (arrows). Genotype as in B.