Fig. 6. Aberrant localization of Syph cargos in syph-deficient embryos. (A-C) High-magnification dorsal view of embryos expressing EB1-EYFP under the control of the En-Gal4 driver (striped pattern) that were injected with buffer alone (A) or with Syph dsRNA (B,C) undergoing DC. Note trafficking of EB1-EYFP LE cell filopodia (brackets) in control embryos (A) that is mostly absent from Syph-deficient embryos (bracket, B; C). Syph-deficient embryos exhibiting the strongest phenotypes also show disrupted EB1-EYFP trafficking within the LE and more ventrally located cells (C). See Movie 4 in the supplementary material. (D-G'') Actin expression (as visualized by phalloidin staining; D-E'') and -tubulin expression (F-G'') in buffer-injected (D-D''; F-F'') and Syph dsRNA-treated (E-E''; G-G'') nuclear-GFP-expressing stage 15 embryos. (H-I') -tubulin expression in wild-type (H,H') and Syph dsRNA-treated (I,I') S2R+ cells. Note reduction of cellular extensions and disruption of MT lattice networks in the Syph RNAi-treated cells. Scale bars: 10 µm in A-C; 20 µm in D-I'.