Fig. 1. Posterior patterning is disrupted in embryos derived from wol germline clones. Anterior is to the left in all figures. Genotypes, RNA probes and antigens are indicated. (A-C) Cuticle preparations of Drosophila embryos prior to hatching. Some posterior denticle belts are missing and the remainder are curled-up in wol embryos, as compared with wild type (wt). (D-I) Ventrolateral views of embryos undergoing germband elongation. Every other en stripe is missing in the posterior of wol embryos. (G,H) Eve protein (brown) labels the odd-numbered en stripes (blue). Arrowheads point to en stripes 7 and 9. (I) Posterior en stripes are missing in embryos from cad germline clones. (J-N) Lateral views of pre-cellular embryos showing changes in gt and kni expression in wol and cad embryos as compared with wild type. Arrow in N indicates a shift in position of the kni stripe towards posterior. (O-R) Maternal cad mRNA is present at similar levels in wild-type and wol mutant embryos, whereas the Cad protein gradient is reduced in wol embryos (R).