Fig. 1. Deduced amino acid sequences of OBE1 and OBE2, and subcellular localization of the OBE1 protein. (A) Alignment of the predicted amino acid sequences of OBE1 and OBE2. Identical amino acid residues and similar amino acid residues are highlighted by black and gray backgrounds, respectively. The PHD finger domain and coiled-coil domain are underlined with blue and black, respectively. (B) Genomic structure of OBE1 and OBE2. Black boxes represent exons. Positions of the start and termination codons are indicated. T-DNA insertion sites in obe1-1 and obe2-1, and the location of obe2-2 mutation are shown. Arrows represent the positions of the primers used to amplify the OBE1 and OBE2 transcripts. (C,D) Confocal images of root hair cells from 35S::OBE1-GFP (C) and 35S::GFP (D) transgenic plants. (C) The OBE1-GFP fusion protein was localized in the nucleus. (D) The GFP signal was detected throughout the cell.