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Figure 2


Fig. 2. Expression of OBE1 and OBE2. (A) RT-PCR analysis of OBE1 and OBE2 expression in various tissues as shown. Accumulation of EF1{alpha} was used as a control. (B-E) Wild-type embryo specimens of in situ hybridization with OBE1 (B,D) and OBE2 (C) antisense probes and (E) OBE1 sense probe. (B) Four-cell stage embryo. (C) Eight-cell stage embryo. Hybridization signals were detected in the embryo proper but not in the suspensor. (D) OBE1 is expressed in all cells of the heart stage embryo. (E) Control hybridization with an OBE1 sense probe at the heart stage. (F) Expression of the OBE1-GFP fusion protein in the torpedo stage embryo from an OBE1p::OBE1-GFP transgenic plant. Scale bars: 25 µm.