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Figure 6


Fig. 6. MAP kinase ERK acts downstream of NvFGF signalling in apical organ formation. (A) Western blot to detect levels of phosporylated and total ERK. (B-G) In situ hybridisation of 48-hpf planulae. Lateral views, aboral pole to the left. (B-D) Embryos treated with 10 µM UO126 from 20-48 hpf; probes are indicated in the lower left corner. (E-G) In situ hybridisation with a NvFGFa1 probe; treatments are indicated in the upper right corner, inhibitor treatment was carried out from 20-48 hpf.