Fig. 7. Metamorphosis, but not swimming, is affected by the manipulation of FGF signalling. (A,B) Frames from movies of individual 4-dpf planula larvae injected with the morpholino indicated. Total width of the frame is 3 mm; time interval between first and last frame was 1.2 seconds in A and 1.7 seconds in B. (C) Illustration of the effect of the indicated morpholinos on metamorphosis at 12 dpf. Uninjected, n=156; control (ctr) MO, n=133; NvFGFa1 MO, n=98; NvFGFa2 MO, n=85. (D-F) Micrographs showing live animals at 12 dpf; injected morpholinos are indicated. All animals injected with the NvFGFa1 MO are still planula larvae, most of the NvFGFa2 MO-injected animals are primary polyps. (G) Effect of SU5402 treatment on metamorphosis. Late planula stage embryos (5 dpf) were continuously incubated with DMSO or 20 µM SU5402 until 9 dpf. DMSO, n=159; SU5402 treatment, n=165. (H) Schematic model of the autoregulation of FGF pathway components in Nematostella apical organ development.