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Figure 6


Fig. 6. Interaction with nos-2 3'UTR is essential for the translation suppression activity of OMA-2. (A) Sequence of the SubE region. Sequences targeted by substitution analysis in EMSA are boxed and named. (B) Electrophoretic mobility shift by OMA-2 of radiolabeled nos-2 3'UTR bearing the indicated mutations. The first lane in each set is the mobility of RNA in the absence of protein. (C) Expression pattern of GFP:H2B in embryos of transgenic worms carrying the GFP:H2B:nos-2 3'UTR transgene with wild-type sequence (WT) or bearing {Delta}27 mutation.