Fig. 1. Both Notch and Sox3 block neurogenesis. (A-E) Notch1 (A), Hes5 (B), Sox3 (C), Ngn2 (D) and E47 (E) exhibited complementary expression patterns in the embryonic day 4.0 chick spinal cord. (F-H) Expression of the intracellular domain of Notch1 (NICD) (F) or Sox3 (G) for 42 hours significantly reduced the generation of Tuj1⁺ neurons (H). Data are represented as percentage of electroporated cells expressing Tuj1 (mean±s.e.m.). ^**P<0.01 relative to EGFP control transfected cells, Student's t-test. Scale bars: 50 µm.