Fig. 5. PIII injection reduces opV trigeminal placode induction in ovo, but does not alter proliferation, cell death or total cell number. (A) Vehicle or PIII solution was focally injected at st. 8 and placode induction assessed at st. 10. PIII (1 µm) (n=6) resulted in 71% reduction of Pax3 relative to controls (n=7). Concentrations of 2.5 and 5 µm PIII caused 67% and 84% reduction (n=8, n=6), respectively. Error bars are s.e.m. ^*P<0.001. (B) No differences in phospho-histone H3+ were noted between control and 2.5 µm PIII embryos (P>0.05; n=5 each). (C) TUNEL staining revealed no difference in cell death between control and 2.5 µm PIII-injected embryos (P>0.05; n=5 each). (D) Total cell number as assayed by DAPI was not different between control and 2.5 µm PIII-injected embryos (P>0.05; n=5 each).