Fig. 10. Sox9^flox/-; AMH-Cre^tg/+ male mice develop normal testes at E18.5. (A-F) Sox9^flox/+; AMH-Cre^tg/+ male mice were crossed with Sox9^flox/flox; Zp3-Cre^tg/+ female mice to obtain Sox9^flox/-; AMH-Cre^tg/+ embryos. Hematoxylin and Eosin staining showing normal histology of Sox9^flox/-; AMH-Cre^tg/+ testes (D) when compared with control testes of the same age (A). SOX9 was totally deleted in Sertoli cells of mutant testes (E). However, the expression of AMH and β-catenin in Sertoli cells was unaffected upon SOX9 deletion (E,F). Scale bar: 20 µm.