Fig. 2. Catnb^fx/fx; AMH-Cre^tg/+ male mice develop normal testes. (A) Sertoli cell-specific deletion of β-catenin in testes of Catnb^fx/fx; AMH-Cre^tg/+ male mice at E16.5. In control testes, β-catenin protein was detected in both germ cells (left panel, arrowhead) and Sertoli cells (left panel, arrows). In Catnb^fx/fx; AMH-Cre^tg/+ testes, β-catenin protein was undetected in Sertoli cells (right panel, arrows) but its expression in germ cells remained unaffected (right panel, arrowhead). β-Catenin protein was stained by an anti-β-catenin antibody (green) and nuclei were counterstained with DAPI (blue). (B) Normal histology of Catnb^fx/fx; AMH-Cre^tg/+ testes at P0 (e) and 7 weeks (f) compared with control testes of the same age (a and b, respectively). Adult mutant mice expressed normal Sertoli cell marker SOX9 (g) and Leydig cell marker 3β-HSD (h), when compared with control mice (c and d, respectively). Scale bars: 20 µm.