Fig. 3. Stabilization of β-catenin in Sertoli cells of Catnb^lox(e3)/+; AMH-Cre^tg/+ mice. (A-H) β-Catenin protein was stained by an anti-β-catenin antibody (green) and nuclei were counterstained with DAPI (blue). The accumulation of β-catenin in Sertoli cells started from E13.5 in a few Sertoli cells (D, arrow) and expanded to more Sertoli cells at E14.5 (F, arrows). At E15.5, almost all Sertoli cells in the testicular cord were affected (H, arrows). The stabilized β-catenin was localized both in cytoplasm and nucleus in Sertoli cells. Scale bars: 20 µm.