Fig. 6. Disruption of testicular cords and loss of germ cells in Catnb^lox(e3)/+; AMH-Cre^tg/+ testes between E15.5 and P0. (A-L) Progressive tubule development in control testes from E15.5 to P0 (low magnification, A,C,E; high magnification, G,I,K), and progressive disruption of cord architecture (arrowheads) in Catnb^lox(e3)/+; AMH-Cre^tg/+ testes (low magnification, B,D,F; high magnification, H,J,L), shown by Hematoxylin and Eosin staining. (M-R) Germ cells (GCNA1 positive) located within tubules of control testes (M,O,Q), but located outside of the tubules in Catnb^lox(e3)/+; AMH-Cre^tg/+ testes from E15.5 (arrow, N,P), and very few germ cells remained by P0 (R). (S-X) Leydig cells (3β-HSD-positive) scattered in interstitial spaces of control testes (S,U,W); masses of Leydig cells were found in the interstitial spaces of the mutant testes (T,V,X). Scale bars: 60 µm in A-F; 20 µm in G-X.