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Figure 4


Fig. 4. The AV cushions do not require Hh signaling for normal AV septation. (A) TnT-Cre is expressed in the myocardium as detected by R26R β-galactosidase activity in whole-mount (A) or in sagittal section (A') at E10.5. (B) Ablation of Smo with TnT-Cre results in normal AV septation (E18.5). (C) Tie2-Cre is expressed in all endothelial cells and derivatives, including AV cushion mesenchyme (C,C'). (D) Tie2-Cre; Smoflox/- embryos have normal AV septation (E18.5). (E,F) Simultaneous ablation of Smo using both Tie2-Cre and TnT-Cre (reported in E,E') does not induce AV septation defects (F, E14.5 transverse section). A, atria; IAVC, inferior atrioventricular cushion; LV, left ventricle; MV, mitral valve; RV, right ventricle; SAVC, superior atrioventricular cushion; TV, tricuspid valve; V, ventricle.