Fig. 2. Q-RT-PCR confirms post-meiotic transcription. (A) Individual cyst Q-RT-PCR assay schematic. (B) Cysts used for RNA extraction, from primary spermatocytes (I1, I2) and round spermatids (R1, R2; scale bars: 25 µm), and from elongating spermatids (0.19-1.89 mm; scale bars: 100 µm). (C,D) Control Q-RT-PCRs. (E-H) Peak of expression in spermatids confirms post-meiotic transcription of comet and cup genes. hale (E) and schuy (F) were expressed in more bundles than were d-cup (G) and sunz (H).