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Figure 3


Fig. 3. Comet and cup transcription precedes the histone-protamine switch. Bars above graphs and colours within graphs indicate H2A-mRFP (histone, red) and Mst35Ba-EGFP (protamine, green; overlap orange/yellow) detection. (A-C) Control Q-RT-PCRs. (D-J) Comet and cup Q-RT-PCR expression profiles show that expression initiates in cysts negative for protamine. (K) Nuclear fluorescence in elongating cysts.