Fig. 4. Edema provoked by Xc-Myc knockdown is associated with massive hemorrhage and impaired expression of X-ERG, Scl and Prox1. (A) The vascular networks of uninjected (Uninj) and Xc-Myc morpholino (Mo)-injected embryos were visualized by staining blood cells with benzidine. Hemorrhagic areas are present throughout body proper of Mo-injected embryos. H, heart; aa, aortic arches; vvn, vitelline vein network; pcv, posterior cardinal vein; da, dorsal aorta. (B) The effects of Xc-Myc knockdown on the expression of genes involved in vascular development was assessed by qRT-PCR in control (dark blue bars) and Xc-Myc morpholino-injected (light blue bars) embryos at stage 28. Expression was normalized to Gapdh.