Fig. 6. Slug or Twist rescue the vascular defects provoked by Xc-Myc knockdown. (A) The percentage of embryos that appeared normal (dark-blue bars) or showed edema associated with hemorrhage (light-blue bars) after injection of Xc-Myc morpholino alone or mixed with Xc-Myc-Mut, wild-type Xc-Myc, Slug, Twist or β-galactosidase mRNAs. (B) Representative images of the results in A. showing the rescue of the Xc-Myc knockdown phenotype by Slug or Twist. (C) One blastomere of a two-cell stage embryo was injected with Slug morpholino. In situ hybridization at the neural tube stage using a probe specific for Xc-Myc showed that knockdown of Slug indeed affects Xc-Myc expression in the region corresponding to the neural crest (i-iii). On the injected side (black arrowheads) an accumulation of Xc-Myc-expressing cells is evident, while on the uninjected side cells expressing Xc-Myc have started to migrate (red arrow). A deviation in the body axis is also evident (ii,iii). Despite affecting Xc-Myc expression during neurulation, knockdown of Slug in two-cell embryos is not sufficient to cause the edema and hemorrhagic phenotype (iv,v), as observed upon Xc-Myc knockdown using Xc-Myc morpholino.