Fig. 7. Xc-Myc or XSlug/XTwist targeted knockdown in lateral plate mesoderm but not the neural crest lineage, provokes edema and hemorrhage. (A) Map showing the blastomeres targeted for knockdown of Xc-Myc or XSlug/XTwist in predominantly neural crest (V1.2) or lateral plate precursors (D2.1 or V2.1). Correct targeting of the morpholinos is shown in the next three images taken at neurula and tailbud when neural crest cells start migrating. NC, neural crest; LPM, lateral plate mesoderm; Neph, nephrotome. (B,C) Embryos were injected with a mixture of either Xc-Myc morpholino (B) or XSlug/XTwist morpholino (C), and were dextran fluorescently labeled with rhodamine. At stage 43, embryos were stained with benzidine to reveal the location of blood. Knockdown of Xc-myc or XSlug/XTwist within the lateral plate mesoderm (3rd and 4th column), but not the neural crest (2nd column), provoked edema (arrows) and hemorrhagic (arrowheads) phenotypes. Levels of blood in the V2.1 injected embryos (4th column) are significantly lower (dashed arrow).