Fig. 2. Expression pattern of the HRP-Grk fusion protein. (A) The schematic of the HRP-Grk fusion protein. SP, signal peptide; EGF, EGF repeat motif; TM, transmembrane domain; Cyt, cytoplasmic domain. (B-M) The localization of HRP-Grk was detected by the anti-HRP antibody (green), and the egg chamber organization was identified by phalloidin staining (red). The wild-type egg chambers served as a negative control (B). HRP staining in the oocyte at stage 7 (C), stage 8 (D) and stage 9 (E) showed similar patterns to endogenous Gurken staining. The punctate HRP signal was detected not only in the dorsal (arrows in F and G) follicle cells but also in the ventral follicle cells (arrows in H,I). These punctate signals were markedly reduced at a non-permissive temperature in shibire mutant background (J-M). (N-Q) Some HRP signal (red) in follicle cells colocalized with an early endosomal marker, GFP-Rab5, in the dorsal (N,N',O) and ventral (P,P',Q) follicle cells (arrows indicate punctate signals that overlapped in merged images). Anterior of the egg chamber is towards the left and dorsal side faces upwards. Scale bars: 20 µm in B-E; 10 µm in F-Q.