Fig. 3. The Grk-Egfr complex is internalized in follicle cells through Rab5-Rab7 endocytic pathways. (A-C) Clonal analysis of mosaic egg chambers shows that the anti-Egfr antibody staining (red) is specific as it is absent from the mutant cells. (D-F') HRP-Grk (red) was colocalized with Egfr (green) in dorsal follicle cells. (G-I) Punctate HRP signals (red) were much less abundant in the top/Egfr mutant follicle cells (star indicates non-GFP cells) compared with adjacent normal cells (arrows indicate punctate HRP signals in wild-type cells). (J-O) Internalized Egfr (red) was colocalized with Rab5 (J-L) and Rab7 (M-O) in the follicle cells. Oo, oocyte; arrows indicate punctate signals that overlapped in merged images (D-F,J-L,M-O); the bracket area was enlarged in F',L',O'. Scale bars: 10 µm.