Fig. 5. Overexpression of CblL altered Gurken distribution. Anterior of the egg chamber is towards the left and dorsal side faces upwards. (A-C) Gurken expression pattern (green) at late stage 9 egg chambers was altered when Cbl was overexpressed. In wild-type egg chambers, Gurken accumulated in the dorsal anterior corner (A). Slightly reduced expression of Gurken was detected in egg chambers with overexpression of CblS driven by GR1-Gal4 (B). Significantly reduced expression of total Gurken in egg chamber in which CblL was overexpressed (C). (D) The percentage of late stage 9 egg chambers that had normal Gurken protein expression. ^*P>0.05; ^**P<0.01. (E-H) The increased HRP-Grk signal (green) in dorsal follicle cells (E) was colocalized with CblL, which was ectopically expressed by EQ-Gal4 and stained with 8C4 antibody (F, red). (H) The composite image of several confocal sections of an egg chamber with an HRP-grk transgene and CblL overexpression. (I-K) Some of these HRP-Grk signals were also colocalized with Rab5 (I), Rab7 (J) and Egfr (K). Arrows indicate punctate signals that overlapped in merged images. The bracket area is enlarged in E',F',G',I',J',K'. Scale bars: 40 µm in A-C; 20 µm in E-J; 10 µm in K.