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Figure 2


Fig. 2. Delayed occurrence of AChR clustering in the absence of the {gamma}-subunit. (A-F) Whole-mount diaphragm muscles (E16.5-E18.5) labeled with Texas Red-conjugated {alpha}-bungarotoxin. AChR clusters were detected in the {gamma}-/- muscles (arrowheads in D-F), although they were less numerous and appeared dimmer than those observed in the wild-type muscle (arrowheads in A-C). (G) Comparison of the fluorescence intensity (mean gray value), area, perimeter and Feret's diameter (the length of the greatest axis) of individual AChR clusters in the {gamma}-/- muscles with those of the wild-type muscles. *P<0.05 (Student's t-test), Scale bar: 50 µm.