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Figure 3


Fig. 3. Expression of the AChR {epsilon}-subunit in embryonic muscles. (A) RT-PCR analyses in wild-type and {gamma}-/- embryos (E13.5, E15.5, E16.5 and E18.5). In both wild-type and {gamma}-/- muscles, the expression of the {alpha}-subunit and GAPDH was readily detectable from E13.5 to E18.5, but the expression of {epsilon}-subunit was detectable only after E16.5 (*), and its level of expression was sharply increased at E18.5 (**). (B) Quantitative real-time PCR analysis using specific TaqMan probes for {epsilon}-subunit gene. The expression levels of {epsilon}-subunit gene in both wild-type and {gamma}-/- muscles (E18.5) were similar (wild-type, 3.1±2.9%, n=3 embryos; {gamma}-/-, 3.4±2.7%, n=3 embryos), both at ~3% of level compared with the adult muscle (100%).