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Figure 6


Fig. 6. Progressive accumulation of synaptic vesicle protein at the nerve terminals. Whole-mount diaphragm muscles (E18.5) were double-labeled with synaptotagmin 2 (Syt2) antibody (A,D) and Texas-Red-conjugated {alpha}-bgt (B,E). The merged images are shown in C and F. In both wild-type (A) and {gamma}-/- muscles (D), individual nerve terminals (arrowheads in A,D) were heavily labeled by Syt2 antibody. AChR clusters are present in the end-plates of the {gamma}-/- muscles (arrowheads in E), but they are less intensely labeled by {alpha}-bgt, compared with those observed in the wild-type muscles (arrowheads in B). Merged images show that nerve terminals are juxtaposed with AChR clusters in both {gamma}-/- (arrowheads in F) and wild-type muscles (arrowheads in C); however, there were marked increases of nerve sprouting in the {gamma}-/- muscles (arrows in D,F), compared with those in the wild-type muscle (arrows in C). Scale bar: 50 µm in A-F.