Fig. 8. Localization of MuSK in the absence of the -subunit. Whole-mount E15.5 diaphragm muscles from the ventral costal region (A-D) and sternal region (E-H) of wild-type (A-B,E-F) and ^-/- (C-D,G-H) embryos were doubly labeled with MuSK antibody and -bungarotoxin. In wild-type muscles, MuSK staining (arrowheads in B and F) was detected at the central region of the muscle, corresponding to AChR clusters labeled by -bungarotoxin (arrowheads in A,E). In the ^-/- muscles, MuSK staining was also seen at the central region of the muscle (arrows in D,H), despite the lack of AChR clustering (C,G). Scale bar: 10 µm.