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Figure 4


Fig. 4. cort mRNA is a meiotic target of Wisp. (A) PAT assays measuring cort poly(A) tail lengths in Drosophila egg chambers of progressive stages from wild-type and wispKG5287 ovaries. sop mRNA was used as a control. (B) Western blots showing that Cort protein levels are reduced in wispKG5287 stage 14 oocytes as compared with wild type. Protein extracts were from 30 egg chambers at stage 9-10 and from 15 oocytes at stage 14. (C) Western blots showing that CycA levels are higher in the ovaries and stage 14 oocytes of wispKG5287 than of wild-type. Protein extracts were from 0.5 ovary and 20 oocytes at stage 14. {alpha}-tubulin was used as a loading control in B and C.