Fig. 3. Co-precipitation of TTG1-cMYC with HA-GL3-6His and GL1-YFP-6His from
seedling extracts. His-Select Ni columns were used to pull down
6His-tagged fusion proteins. Input and eluted proteins were separated by
SDS-PAGE gels in the order labeled. Membranes were probed with anti-cMYC mAb.
In vivo interaction between TTG1 and GL3 is indicated in A and between
TTG1 and GL1 in B.