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Figure 4


Fig. 4. Speckled nuclear distribution of GL3 in the leaf epidermis of ttg1 and gl1 mutants. Confocal images of (A) gl3/pGL3::GL3-YFP, (B) ttg1/pGL3::GL3-YFP, (C) ttg1/pGL1::GL1-YFP-cM, (D) gl1/pGL3::GL3-YFP and (E) gl1/pGL3::GL3-YFP. GL3-YFP is unevenly distributed and forms nuclear speckles in leaf epidermal cells of ttg1 and gl1 mutants (B,D). GL1-YFP formed only a couple of speckles in nuclei in occasional leaf epidermal cells (C). Uniform GL3-YFP distribution in gl3 leaf epidermal cells (A), and gl1 root epidermal cells (inset picture shows GL3::GL3-YFP accumulation in hairless cell files forming wild type-looking stripes) (D). Scale bars: 10 µm.