Fig. 6. The expression of Tlr2 and Tlr4 mRNA and the role of cytokines/chemokines in human fertility. (A) The expression of Tlr2 and Tlr4 mRNA in cumulus cells of human COCs. The cumulus cells were recovered from COCs after in vitro fertilization. (B) The relationship between the amount of secreted CCL cytokines and fertilization in vitro. The cytokines present in the media were analyzed by using the Bio-Plex Human Cytokine 9-Plex Panel. Y, all oocytes were successfully fertilized; N, more than half of the oocytes were not fertilized. P values are for Y versus N.