Fig. 5. Reduction in the number of progenitors in the dentate gyrus of
Ngn2 mutant mice. (A-B') Hippocampal sections of
E16.5 (A,A') and E18.5 (B,B') Ngn2KIGFP/+
(A,B) or Ngn2KIGFP/GFP (A',B') brains
immunolabelled for GFP, Ki67 and BrdU following a 30-minute pulse. Arrows
indicate double labelled cells.
(A1,A'1,B1-B3,B'1-B'3)
High magnification of the areas outlined in A-B'.(C,E)
Histograms representing the total number of BrdU-containing cells (C) or
Ki67-expressing cells (E) within the different matrices of
Ngn2KIGFP/+ (blue bars) and
Ngn2KIGFP/GFP (red bars) DG. The number of
BrdU+ progenitors is reduced in the three matrices of the mutant
DG. The fimbria, which is not affected by the loss of Ngn2, is used
as a control. (D) Histogram representing the percentage of
GFP-expressing cells that have incorporated BrdU. (F) Histogram
representing the ratio of BrdU- and Ki67-expressing cells.
*P
0.05, **P0.01. Scale bars:
50 µm.