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Figure 4


Fig. 4. Talin 1 is not essential for costamere assembly. (A-D) Longitudinal and (E-L) cross-sections of muscle from 6-month-old wild-type and Tln1HSA-CREko mice were stained with antibodies to costameric proteins and ECM glycoproteins and analyzed by immunofluorescence microscopy. The integrin β1-subunit, {alpha}-actinin, vinculin, dystrophin, collagen type IV and laminin were localized normally in the mutants. (M-P) Analysis of muscle by transmission electron microscopy. Sarcomere organization was not affected in Tln1HSA-CREko mice (M,N), but sarcomeres occasionally appeared hypercontracted (O,P). The Z-band was always detectable (O,P, arrows), but the A band (O,P, asterisk) was sometimes not evident in contracted muscle fibers. Scale bars: 200 µm in A-L; 2 µm in M,N; 1 µm in O,P.