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Figure 5


Fig. 5. Expression and phosphorylation of talin 1-associated proteins. (A-C) Extracts from wild-type and Tln1 mutant gastrocnemius muscle were analyzed by western blotting. No changes were observed in levels of FAK (A), Erk1/2 (B, ERK), β1 integrin and vinculin (C). Phosphorylation of FAK at Tyr397 (A, P-FAK) and of Erk1/2 (B, P-ERK) were unaffected. Membranes were probed with {alpha}-tubulin antibodies as a loading control. (D) Protein expression was quantified by densitometry (n≥3; relative expression levels ±s.e.). No statistically significant difference was observed in expression (P-values: β1 integrin, 0.238; vinculin, 0.146; FAK, 0.223; P-FAK, 0.153; ERK, 0.892; P-ERK, 0.165).