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Figure 1


Fig. 1. Branching morphogenesis of the Drosophila tracheal system during embryonic development. Stills from time-lapse movies showing Drosophila embryos expressing either (A) His2AvD::mRFP ubiquitously or (B-D) GFP::Actin in the tracheal system (see Movie 1 in the supplementary material). (A) Stage 11 of embryonic development. Tracheal cells invaginate (arrowheads) and form tracheal sacs during the initial phase of germ band retraction. (B) Stage 12 of embryonic development. Tracheal sacs extend branches in stereotyped directions (asterisks). (C) Stage 14 of embryonic development. Tracheal branches elongate. (D) Stage 15 of embryonic development. Some branches are fusing to form an interconnected network of tracheal tubes (arrowheads). DBs, dorsal branches; DT, dorsal trunk; TCs, transverse connectives. Anterior is to the left and dorsal to the top. Scale bars: 100 µm.