Fig. 2. EKLF expression is initiated prior to erythroid commitment during EB differentiation. (A) GFP expression in relation to hematopoietic marker gene expression between days 2 and 8 of mouse EB differentiation. Levels of GFP, Eklf, Gata1, Gata2 and globin βH1 (bh1) mRNA in samples harvested at the indicated day after EB differentiation were monitored by qRT-PCR and normalized to 18S rRNA. Values are presented relative to the maximum expression level per gene, which was set to 1. (B) Flow cytometric analysis of GFP expression at day 5.5 of differentiation in EBs derived from Peklf-GFP ES cells. (C) Percentages of progenitor-derived colonies from sorted GFP⁺ EB cells in one representative experiment.